Ameliorative effects of melatonin and zinc oxide nanoparticles treatment against adverse effects of busulfan induced infertility in male albino mice

Testicular damage is one of the most hazardous effects as it’s associated with azoospermia. Busulfan (Bu) a highly toxic chemotherapeutic drug that affects testis. Thirty male Swiss albino mice divided into six groups 5 animals each. Control (oral 0.9% saline daily for 75 days); Mel (20 mg/kg/day orally 30 ZnO NPs (5 i.p. BU (single injection 40 mg/kg and then left 45 + dose days followed by 20 days, days). Preparation Characterization NPs. Specimens from testis prepared ultrastructural investigations using TEM after Masson’s trichrome toluidine blue staining. induced histological Moreover, present results could be concluded or can protect testicular tissue against alterations its antioxidant anti-apoptotic effects.